human clear cell ovarian carcinoma cell es 2  (ATCC)

Journal: International Journal of Medical Sciences

Article Title: Identification and Validation of Prognostic Markers for Endometriosis-Associated Ovarian Cancer

doi: 10.7150/ijms.97024

Figure Lengend Snippet: Detailed information of GEO and GTEx datasets containing the EMs and EAOC patients.

Article Snippet: Human normal ovarian epithelial cell line IOSE80 was purchased from iCell Bioscience Inc (Shanghai, China), human ovarian clear cell carcinoma cell line ES-2 was purchased from Procell Life Science&Technology (Wuhan,China) and human ovarian endometrioid carcinoma cell line TOV-112D was purchased from FuHeng Biology (Shanghai, China).

Techniques:

Journal: Cancer Informatics

Article Title: Computational Methods for Structure-to-Function Analysis of Diet-Derived Catechins-Mediated Targeting of In Vitro Vasculogenic Mimicry

doi: 10.1177/11769351211009229

Figure Lengend Snippet: (A) In vitro VM in 2 ovarian cancer cell models. Representative capillary-like structure formation at time 0 hour and time 24 hours from ES2 and SKOV3 ovarian cancer cells. (B) Fractal dimension analysis on SKOV3 and ES2 in vitro VM. Fractal dimension, Df = 2 ± 8.0825e-16 was computed for both models. The box-count plots are shown, where the space-filling (red dashed line) denotes the fractal curve filling a 2D unit box to map the recognized VM structures. The actual box-count (blue line) adjusts the space-filling curve to the measured local scaling exponents computed by the algorithm. Here, n(r) is the number of boxes and r is the box size of the structures in the box-counting algorithm. VM indicates vasculogenic mimicry.

Article Snippet: Human SKOV3 ovarian adenocarcinoma cells as well as human ES2 ovarian clear cell carcinoma cells were purchased from the American Type Culture Collection (ATCC), and monolayers cultured as previously described with McCoy’s 5a Modified Medium for ES2 cells (Wisent, 317-010-CL), and DMEM medium (Wisent, 319-005-CL) for SKOV3 cells both containing 10% fetal bovine serum (Life Technologies, 12483-020), 100 U/mL penicillin, and 100 mg/mL streptomycin (Wisent, 450-202-EL).

Techniques: In Vitro

Journal: Cancer Informatics

Article Title: Computational Methods for Structure-to-Function Analysis of Diet-Derived Catechins-Mediated Targeting of In Vitro Vasculogenic Mimicry

doi: 10.1177/11769351211009229

Figure Lengend Snippet: Fractal dimension analysis on ES2 model.

Article Snippet: Human SKOV3 ovarian adenocarcinoma cells as well as human ES2 ovarian clear cell carcinoma cells were purchased from the American Type Culture Collection (ATCC), and monolayers cultured as previously described with McCoy’s 5a Modified Medium for ES2 cells (Wisent, 317-010-CL), and DMEM medium (Wisent, 319-005-CL) for SKOV3 cells both containing 10% fetal bovine serum (Life Technologies, 12483-020), 100 U/mL penicillin, and 100 mg/mL streptomycin (Wisent, 450-202-EL).

Techniques:

Journal: Cancer Informatics

Article Title: Computational Methods for Structure-to-Function Analysis of Diet-Derived Catechins-Mediated Targeting of In Vitro Vasculogenic Mimicry

doi: 10.1177/11769351211009229

Figure Lengend Snippet: (A) Sample images of 2D wavelet analysis of ES2 cells. The wavelet analyses on the C and CG VM images (upper panels) are shown as decomposed by the MATLAB wavelet analyzer application (lower panels) from the original images, where the axes correspond to the pixel sizes. The wavelet decomposition images were analyzed with the Haar wavelet basis at level 3 where the axes correspond to the wavelet coefficients (diagonal coefficient not shown). (B) Mean branching clusters assessed by 2D wavelet analysis of ES2 VM. Histogram statistics from 2D wavelet analysis in ES2 model showing the mean percolation clusters observed with all 8 catechins tested ( P < .0001, r 2 = 0.999 when comparing gallated vs ungallated groups). VM, vasculogenic mimicry; C, catechin; CG, catechin gallate; EC, epicatechin; ECG, epicatechin gallate; EGC, epigallocatechin; EGCG, epigallocatechin-3-gallate; GC, gallocatechin; GCG, gallocatechin gallate.

Article Snippet: Human SKOV3 ovarian adenocarcinoma cells as well as human ES2 ovarian clear cell carcinoma cells were purchased from the American Type Culture Collection (ATCC), and monolayers cultured as previously described with McCoy’s 5a Modified Medium for ES2 cells (Wisent, 317-010-CL), and DMEM medium (Wisent, 319-005-CL) for SKOV3 cells both containing 10% fetal bovine serum (Life Technologies, 12483-020), 100 U/mL penicillin, and 100 mg/mL streptomycin (Wisent, 450-202-EL).

Techniques:

Journal: Cancer Informatics

Article Title: Computational Methods for Structure-to-Function Analysis of Diet-Derived Catechins-Mediated Targeting of In Vitro Vasculogenic Mimicry

doi: 10.1177/11769351211009229

Figure Lengend Snippet: Percolation clustering statistics in ES2 model.

Article Snippet: Human SKOV3 ovarian adenocarcinoma cells as well as human ES2 ovarian clear cell carcinoma cells were purchased from the American Type Culture Collection (ATCC), and monolayers cultured as previously described with McCoy’s 5a Modified Medium for ES2 cells (Wisent, 317-010-CL), and DMEM medium (Wisent, 319-005-CL) for SKOV3 cells both containing 10% fetal bovine serum (Life Technologies, 12483-020), 100 U/mL penicillin, and 100 mg/mL streptomycin (Wisent, 450-202-EL).

Techniques:

Journal: Cancer Informatics

Article Title: Computational Methods for Structure-to-Function Analysis of Diet-Derived Catechins-Mediated Targeting of In Vitro Vasculogenic Mimicry

doi: 10.1177/11769351211009229

Figure Lengend Snippet: Horizontal wavelet coefficients. Higher coefficients generally imply greater levels of cluster connectivity. The globally largest level 3 horizontal mean coefficients of each catechin-treated group observed in the 2D wavelet analysis of ES2 cells. The coefficients are a global measure of the level of convolution at which the branching clusters identified in can be reconstructed using the Haar wavelet basis. C, catechin; CG, catechin gallate; EC, epicatechin; ECG, epicatechin gallate; EGC, epigallocatechin, EGCG, epigallocatechin-3-gallate; GC, gallocatechin; GCG, gallocatechin gallate.

Article Snippet: Human SKOV3 ovarian adenocarcinoma cells as well as human ES2 ovarian clear cell carcinoma cells were purchased from the American Type Culture Collection (ATCC), and monolayers cultured as previously described with McCoy’s 5a Modified Medium for ES2 cells (Wisent, 317-010-CL), and DMEM medium (Wisent, 319-005-CL) for SKOV3 cells both containing 10% fetal bovine serum (Life Technologies, 12483-020), 100 U/mL penicillin, and 100 mg/mL streptomycin (Wisent, 450-202-EL).

Techniques: